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For recent list of publications, please visit Publication list (PubMed) or Scholar page.


Chowdhury, S., Ramchandran, R., Palecek, S.P., Acevedo-Acevedo, S., Bishop, E. (2023). Sucrose nonfermenting-related kinase expression is related to a metabolic switch in ovarian cancer cells that results in increased fatty acid oxidation. Cancer Investigation.

Chang, H., Chang, D.H., Stamoulis, A.G., Huber, G.W., Lynn, D.M., Palecek, S.P., Dumesic, J.A. (2023). Controlling the toxicity of biomass-derived difunctional molecules as potential pharmaceutical ingredients for specific activity toward microorganisms and mammalian cells. Green Chemistry.

Floy, M.E., Shabnam, F., Givens, S.E., Patil, V.A., Ding, Y., Li, G., Roy, S., Raval, A.N., Schmuck, E.G., Masters, K.S., Ogle, B.M., Palecek, S.P. (2023). Identifying molecular and functional similarities and differences between human primary cardiac valve interstitial cells and ventricular fibroblasts. Front. Bioeng. Biotechnol..


Nishihara, H., Perriot, S., Gastfriend, B.D., Steinfort, M., Cibien, C., Soldati, S., Matsuo, K., Guimbal, S., Mathias, A., Palecek, S.P., Shusta, E.V., Pasquier, R.D., Engelhardt, B. (2022). Intrinsic blood–brain barrier dysfunction contributes to multiple sclerosis pathogenesis Oxford University Press.

Thirugnanam, K., Prabhudesai, S., Why, E.V., Pan, A., Gupta, A., Foreman, K., Zennadi, R., Rarick, K.R., Nauli, S.M., Palecek, S.P., Ramchandran, R. (2022). Ciliogenesis mechanisms mediated by PAK2-ARL13B signaling in brain endothelial cells is responsible for vascular stability Biochemical Pharmacology.

Zhang, J., Gregorich, Z.R., Tao, R., Kim, G.C., Lalit, P.A., Carvalho, J.L., Markandeya, Y., Mosher, D.F., Palecek, S.P., Kamp, T.J. (2022). Cardiac differentiation of human pluripotent stem cells using defined extracellular matrix proteins reveals essential role of fibronectin eLife.

Floy, M.E., Shabnam, F., Palecek, S.P. (2022). Directed differentiation of human pluripotent stem cells to epicardial-derived fibroblasts STAR protocols.

Floy, M.E., Shabnam, F., Simmons, A.D., Bhute, V.J., Jin, G., Friedrich, W.A., Steinberg, A.B., Palecek, S.P. (2022). Advances in Manufacturing Cardiomyocytes from Human Pluripotent Stem Cells. J Annual Review of Chemical and Biomolecular Engineering.

Foreman, K.L., Palecek, S.P., Shusta, E.V. (2022). Human In Vitro Blood-Brain Barrier Models Derived from Stem Cells. Springer, Drug Delivery to the Brain.

Kapur, A., Mehta, P., Simmons, A.D., Ericksen, S.S., Mehta, G., Palecek, S.P., Felder, M., Stenerson, Z., Nayak, A., Dominguez, J.M.A., Patankar, M., Barroilhet, L.M. (2022). Atovaquone: an inhibitor of oxidative phosphorylation as studied in gynecologic cancers. J Cancers.

Agarwal, H., Quinn, L.J., Walter, S.C., Polaske, T.J., Chang, D.H., Palecek, S.P., Blackwell, H.E., Lynn, D.M. (2022). Slippery antifouling polymer coatings fabricated entirely from biodegradable and biocompatible components ACS Appl. Mat..


Ding, Y., Shusta, E.V., Palecek, S.P. (2021). Integrating in vitro disease models of the neurovascular unit into discovery and development of neurotherapeutics. Curr Opin Biomed Eng.

Floy, M.E., Dunn, K.K., Mateyka, T.D., Reichardt., I.M., Steinberg, A.S., Palecek, S.P. (2021). Direct coculture of human pluripotent stem cell-derived cardiac progenitor cells with epicardial cells induces cardiomyocyte proliferation and reduces sarcomere organization. J Mol Cell Cardiol.

Bayne, E.F., Simmons, A.D., Roberts, D.S., Zhu, Y., Aballo, T.J., Wancewicz, B., Palecek, S.P., Ge, Y. (2021). Multiomics Method Enabled by Sequential Metabolomics and Proteomics for Human Pluripotent Stem-Cell-Derived Cardiomyocytes. J Proteome Res.

Jin, G., Floy, M.E., Simmons, A.D., Arthur, M.M., Palecek, S.P. (2021). Spatial Stem Cell Fate Engineering via Facile Morphogen Localization. Adv Healthc Mater.

Grancharova, T., Gerbin, K.A., Rosenberg, A.B., Roco, C.M., Arakaki, J.E., DeLizo, C.M., Dinh, S.Q., Donovan-Maiye, R.M., Hirano, M., Nelson, A.M., Tang, J., Theriot, J.A., Yan, C., Menon, V., Palecek, S.P., Seeling, G., Gunawardane, R.N. (2021). A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes. Sci Rep.

Floy, M.E., Givens, S.E., Matthys, O.B., Mateyka, T.D., Kerr, C.M., Steinberg, A.B., Silva, A.C., Zhang, J., Mei, Y., Olge, B.M., McDevitt, T.C., Kamp, T.J., Palecek, S.P. (2021). Developmental lineage of human pluripotent stem cell-derived cardiac fibroblasts affects their functional phenotype. FASEB J.

Wang, L., Sievert, D., Clark, A.E., Lee, S., Federman, H., Gastfriend, B.D., Shusta, E.V., Palecek, S.P., Carlin, A.F., Gleeson, J.G. (2021). A human three-dimensional neural-perivasuclar 'assembloid' promotes astrocytic deevelopment and enables modeling of SARS-CoV-2 neuropathology. Nat Med.

Nishihara, H., Gastfriend, B.D., Kasap, P., Palecek, S.P., Shusta, E.V., Engelhardt, B. (2021). Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions. STAR Protoc.

Lee, M.S., Stebbins, M.J., Jiao, H., Huang, H.C., Leiferman, E.M., Walczak, B.E., Palecek, S.P., Shusta, E.V., Li, W.J. (2021). Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration. Sci Adv..

Gastfriend, B.D., Foreman, K.L., Katt, M.E., Palecek, S.P., Shusta, E.V. (2021). Integrative Analysis of the Human Brain Mural Cell Transcriptome. J Cereb Blood Flow Metab.

Simmons, A.D. and Palecek, S.P. (2021). Rational, Unbiased Selection of Reference Genes for Pluripotent Stem Cell-Derived Cardiomyocytes. Tissue Eng Part C Methods.

Gleeson, J., Wang, L., Sievert, D., Clark, A., Federman, H., Gastfriend, B., Shusta, E.V., Palecek, S.P., Carlin, A. (2021). A Human 3D Neural Assembloid Model for SARS-CoV-2 Infection. Res Sq.

Gastfriend, B.D., Stebbins, Du, F., Shusta, E.V., Palecek, S.P. (2021). Generation of Brain Pericyte-Like Cells from Human Pluripotent Stem Cell-Derived Neural Crest. Curr Protoc.


Acevedo-Acevedo, S., Millar, D.C., Simmons, A.D., Favreau, P. Cobra, P.F., Skala, M., Palecek, S.P. (2020). Metabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture. Sci Rep.

Nishihara, H., Gastfriend, B.D., Soldati, S., Perriot, S., Mathias, A., Sano, Y., Shimizu, F., Gosselet, F., Kanda, T., Palecek, S.P., Du Pasquier, R., Shusta, E.V., Engelhardt, B. (2020). Advancing human induced pluripotent stem cell-derived blood-brain barrier models for studying immune cell interactions. FASEB J.

Song, H.W., Foreman, K.L., Gastfriend, B.D., Kuo, J.S., Palecek, S.P., Shusta, E.V. (2020). Transcriptomic comparison of human and mouse brain microvessels. Sci Rep.

Lee, W.H., Bhute, V.J., Higuchi, H., Ikeda, S., Palecek, S.P, Ikeda, A. (2020). Metabolic alterations caused by the mutation and overexpression of the Tmem135 gene. Exp Biol Med (Maywood).

Floy, M.E., Mateyka, T.D., Foreman, K.L., Palecek, S.P. (2020). Human pluripotent stem cell-derived cardiac stromal cells and their applications in regenerative medicine. Stem Cell Res.


Canfield, S.G., Stebbins, M.J., Faubion, M.G., Gastfriend, B.D., Palecek, S.P., Shusta, E.V. (2019). An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons. Fluids Barriers CNS.

Rodríguez Lόpez, A.L., Lee, M.R., Wang, N.B., Dunn, K.K., Sanchez, H., Raman, N., Andes, D.R., Lynn, D.M., Palecek, S.P. (2019). Small-Molecule Morphogenesis Modulators Enhance the Ability of 14-Helical β-Peptides to Prevent Candida albicans Biofilm Formation. Antimicrob Agents Chemother.

Dunn, K.K., Reichardt, I.M., Simmons, A.D., Jin, G., Floy, M.E., Hoon, K.M., Palecek, S.P. (2019). Coculture of Endothelial Cells with Human Pluripotent Stem Cell-Derived Cardiac Progenitors Reveals a Differentiation Stage-Specific Enhancement of Cardiomyocyte Maturation. Biotechnol. J.

Stebbins, M.J., Gastfriend, B.D., Canfield, S.G., Lee, M.S., Richards, D., Faubion, M.G., Li, W.J., Daneman, R., Palecek, S.P., Shusta, E.V. (2019). Human pluripotent stem-cell derived brain pericyte-like cells induce blood-brain barrier properties. Sci Adv.

Rodríguez Lόpez, A.L., Lee, M.R., Ortiz, B.J., Gastfriend, B.D., Whitehead, R., Lynn, D.M., Palecek, S.P. (2019). Preventing S. aureus biofilm formation on titanium surfaces by the release of antimicrobial β-peptides from polyelectrolyte multilayers. Acta Biomater.

Qian., T., Hernday, S.E., Bao, X., Olson, W.R., Panzer, S.E., Shusta, E.V., Palecek, S.P. (2019). Directed Differentiation of Human Pluripotent Stem Cells to Podocytes under Defined Conditions. Sci Rep.

Wesley, U.V., Bhute, V.J., Hatcher, J.F., Palecek, S.P., Dempsey, R.J. (2019). Local and systemic metabolic alterations in brain, plasma, and liver of rats in response to aging and ischemic stroke, as detected by nuclear magnetic resonance (NMR) spectroscopy. Neurochem Int.

Lee, M.R., Raman, N., Ortiz-Bermúdez, P., Lynn, D.M., Palecek, S.P. (2019). 14-Helical β-Peptides Elicit Toxicity against C. albicans by Forming Pores in the Cell Membrane and Subsequently Disrupting Intracellular Organelles. Cell Chem. Biol.


Ayuso, J., Gillete, A., Lugo-Cintrόn, Acevedo-Acevedo, S., Gomez, I., Morgan, M., Heaster, T., Wisinski, K.B., Palecek, S.P., Skala, M.C., Beebe, D.J. (2018). Organotypic microfluidic breast cancer model reveals starvation-induced spatial-temporal metabolic adaptations. EBioMedicine.

Acevedo-Acevedo, S., Millar, D.C., Palecek, S.P. (2018). Elucidating the metabolic crosstalk between lymphatic endothelial cells and breast cancer using 1H NMR metabolomics. Cancer Research, 73.

Canfield, S.G., Jin, G., Palecek, S.P., Sampsell T. (2018). Software to improve transfer and reproducibility of cell culture methods. Biotechniques.

Gastfriend B.D., Palecek S.P., Shusta E.V. (2018). Modeling the blood–brain barrier: Beyond the endothelial cells. Current Opinion in Biomedical Engineering, 5:6-12.

Dunn K.K., Palecek S.P. (2018). Engineering Scalable Manufacturing of High-Quality Stem Cell-Derived Cardiomyocytes for Cardiac Tissue Repair. Frontiers in Medicine, 5:110.

Al-Ahmad A.J., Patel R., Palecek S.P., Shusta E.V. (2018). Hyaluronan impairs the barrier integrity of brain microvascular endothelial cells through a CD44-dependent pathway. Journal of Cerebral Blood Flow & Metabolism, doi: 10.1177/0271678X18767748.

Cabral J.M.S., Palecek S.P. (2018). Regenerative Medicine Manufacturing. Biotechnology Journal, 13(2), 1800026

Stebbins M.J., Lippmann E.S., Faubion M.G., Daneman R., Palecek S.P., Shusta E.V. (2018). Activation of RARα, RARγ, or RXRα Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnology Journal, 13(2), 1700093

Piscopo N.J., Mueller K.P., Das A., Hematti P., Murphy W.L., Palecek S.P., Capitini C.M., Saha K. (2018). Bioengineering solutions for manufacturing challenges in CAR T cells. Biotechnology Journal, 13(2), 1700095


Bao X., Bhute V.J., Han T., Qian T., Lian X., Palecek S.P. (2017). Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells. Bioengineering & Translational Medicine, 2(2), 191–201.

Qian T., Maguire S.E., Canfield S.G., Bao X., Olson W.R., Shusta E.V., Palecek S.P. (2017). Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells. Science Advances, 3(11), e1701679.

Kim B.J., Bee O.B., McDonagh M.A., Stebbins M.J., Palecek S.P., Doran K.S., Shusta E.V. (2017). Modeling Group B Streptococcus and Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. mSphere, 1;2(6), e00398-17.

Lee M.R., Raman N., Gellman S.H., Lynn D.M., Palecek S.P. (2017). Incorporation of β-Amino Acids Enhances the Antifungal Activity and Selectivity of the Helical Antimicrobial Peptide Aurein 1.2. ACS Chemical Biology, 12(12), 2975-2980.

Bao X., Lian X., Qian T., Bhute V.J., Han T., Palecek S.P. (2017). Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions. Nature Protocols, 12(9), 1890-1900.

Bhute V.J., Bao X., Palecek S.P. (2017). Advances in Applications of Metabolomics in Pluripotent Stem Cell Research. Current Opinion in Chemical Engineering, 15, 36-43.

Bhute V.J., Bao X., Dunn K.K., Knutson K.R., & ... Palecek S.P. (2017). Metabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes. Theranostics, 7(7), 2078-2091.

Vatine G.D., Al-Ahmad A., Barriga B.K., Svendsen S., Salim A., ... & Svendsen C.N. (2017). Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier. Cell Stem Cell, 20(6), 831-843.

Moon J., Zhou H., Zhang L.S., Tan W., Liu Y., ... & Lum L. (2017). Blockade to pathological remodeling of infarcted heart tissue using a porcupine antagonist. Proceedings of the National Academy of Sciences, 114(7), 1649-1654.

Canfield S.G., Stebbins M.J., Morales B.S., Asai S.W., Vatine G.D., Svendsen C.N., Palecek S.P., Shusta E.V. (2017). An isogenic blood-brain barrier model comprising brain endothelial cells, astrocytes, and neurons derived from human induced pluripotent stem cells. Journal of Neurochemistry, 140(6), 874-888.


Manna U., Raman N., Welsh M.A., Zayas-Gonzalez Y.M., Blackwell H.E., Palecek S.P., Lynn D.M. (2016). Slippery Liquid-Infused Porous Surfaces that Prevent Microbial Surface Fouling and Kill Non-Adherent Pathogens in Surrounding Media: A Controlled Release Approach. Advanced Functional Materials, 26(21), 3599-3611.

Wilson H.K., Faubion M.G., Hjortness M.K., Palecek S.P., Shusta E.V. (2016). Cryopreservation of Brain Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Is Enhanced by Rho-Associated Coiled Coil-Containing Kinase Inhibition. Tissue Engineering Part C: Methods, 22(12), 1085-1094.

Bhute V.J., Ma Y., Bao X., Palecek S.P. (2016). The Poly (ADP-Ribose) Polymerase Inhibitor Veliparib and Radiation Cause Significant Cell Line Dependent Metabolic Changes in Breast Cancer Cells. Scientific Reports, 6:36061.

Cossette S.M., Bhute V.J., Bao X., Harmann L.M., Horswill M.A., & Ramchandran R. (2016). Sucrose Nonfermenting-Related Kinase Enzyme-Mediated Rho-Associated Kinase Signaling is Responsible for Cardiac Function. Circulation: Cardiovascular Genetics, 9(6), 474-486.

Bao X., Lian X., Palecek S.P.. (2016). Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions. Methods in Molecular Biology, 1481, 183-96.

Raman N., Lee M.R., Rodríguez López A.L., Palecek S.P., Lynn D.M. (2016). Antifungal activity of a β-peptide in synthetic urine media: Toward materials-based approaches to reducing catheter-associated urinary tract fungal infections. Acta Biomaterialia, 43, 240-250.

Clark P.A., Al-Ahmad A.J., Qian T., Zhang R.R., ... & Shusta E.V. (2016). Analysis of Cancer-Targeting Alkylphosphocholine Analogue Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model. Molecular Pharmaceutics, 13(9), 3341-3349.

Villa-Diaz L.G., Kim J.K., Laperle A., Palecek S.P., Krebsbach P.H. (2016). Inhibition of Focal Adhesion Kinase Signaling by Integrin α6β1 Supports Human Pluripotent Stem Cell Self-Renewal. Stem Cells, 34(7), 1753-1764.

Raman N., Marchillo K., Lee M.R., Rodríguez López A.L., Andes D.R., Palecek S.P., Lynn D.M. (2016). Intraluminal Release of an Antifungal β-Peptide Enhances the Antifungal and Anti-Biofilm Activities of Multilayer-Coated Catheters in a Rat Model of Venous Catheter Infection. ACS Biomaterials Science & Engineering, 2(1), 112-121.

Hsiao C., Lampe M., Nillasithanukroh S., Han W., Lian X., Palecek S.P. (2016). Human pluripotent stem cell culture density modulates YAP signaling. Biotechnology Journal, 11(5), 662-675.

Bao X., Lian X., Hacker T.A., ... & Palecek S.P. (2016). Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nature Biomedical Engineering, 1: 0003.

Stebbins M.J., Wilson H.K., Canfield S.G., Qian T., Palecek S.P., Shusta E.V. (2016). Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods, 101, 93-102.

Selekman J.A., Lian X., Palecek S.P. (2016). Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small-Molecule Inhibitor of Src Family Kinases. Methods in Molecular Biology, 1307, 319-327.